Kev tshawb fawb ntawm cov kab mob yuav tsum tau ua haujlwm zoo nrog ntau yam khoom siv thiab cov cuab yeej siv. Txhawm rau kom cov kab mob sib kis tau sai npaum li sai tau hauv chav kuaj mob thiab kom muaj peev xwm tswj tau cov haujlwm tseem ceeb, cov khoom siv tshwj xeeb yog siv. Lawv muaj pes tsawg leeg thiab biophysical tej yam kev mob yog haum rau active kev loj hlob ntawm ib tug kab mob kab mob.
Nutrient media. Microbiology thiab lwm yam kev siv
Cov kab mob kab mob loj hlob hauv chav kuaj ntawm Petri cov tais diav uas muaj cov jelly zoo li lossis cov kua dej ib nrab. Cov no yog cov khoom noj khoom haus, cov muaj pes tsawg leeg thiab cov khoom uas yog ze li sai tau rau cov ntuj tsim kom muaj kev loj hlob zoo.
Ib puag ncig zoo li no yog siv hauv kev tshawb fawb microbiological thiab hauv chaw kuaj mob kuaj mob. Kev ua haujlwm tom kawg feem ntau nrog smears ntawm cov kab mob pathogenic lossis cov kab mob ua rau muaj kev cuam tshuam, txoj haujlwm ua haujlwm uas tau txiav txim ncaj qha hauv lub tsev haujlwm.
Natural thiab hluavtawsWednesday
Txoj cai tseem ceeb ntawm kev ua haujlwm nrog cov kab mob yog kev xaiv kom raug ntawm cov khoom noj nruab nrab. Nws yuav tsum ua tau raws li ntau yam, suav nrog cov ntsiab lus ntawm micro thiab macro ntsiab, enzymes, tus nqi tas li ntawm acidity, osmotic siab, thiab txawm tias feem pua ntawm cov pa oxygen hauv huab cua.
Cov khoom noj khoom haus tau muab faib ua ob pawg loj:
- Natural ib puag ncig. Xws li cov khoom xyaw yog npaj los ntawm cov khoom xyaw ntuj. Qhov no tej zaum yuav yog cov dej hauv dej, cov khoom cog, cov quav, zaub, cov ntaub so ntswg thiab cov tsiaj, cov poov xab, thiab lwm yam. Cov cheeb tsam no muaj cov ntsiab lus siab ntawm cov tshuaj ntuj tsim, ntau yam uas txhawb kev loj hlob ntawm cov kab mob kab mob. Txawm hais tias muaj txiaj ntsig zoo, ib puag ncig ntuj tsis tso cai rau kev tshawb fawb tshwj xeeb nrog cov kab mob tshwj xeeb.
- Synthetic media. Lawv txawv nyob rau hauv hais tias lawv cov tshuaj muaj pes tsawg leeg yog paub nyob rau hauv meej ratios ntawm tag nrho cov constituents. Cov xov xwm zoo li no tau npaj rau cov kab lis kev cai tshwj xeeb ntawm cov kab mob, cov metabolism uas paub ua ntej rau tus kws tshawb fawb. Qhov tseeb, vim li no, nws muaj peev xwm npaj xws li ib puag ncig hluavtaws rau kev loj hlob ntawm cov kab mob. Lawv yog siv los tshuaj xyuas cov haujlwm tseem ceeb ntawm cov kab mob. Piv txwv li, koj tuaj yeem paub seb cov tshuaj twg lawv tso rau hauv ib puag ncig thiab ntau npaum li cas. Cov kab mob me me kuj tseem yuav loj hlob hauv cov xov xwm ntuj, tab sis nws tsis tuaj yeem taug qab txhua qhov kev hloov pauv hauv cov muaj pes tsawg leeg vim tsis paub txog thawj feem ntawm cov tshuaj.
Differential-Diagnostic ib puag ncig
Hauv kev ua haujlwm nrog cov kab mob, tsis tsuas yog siv cov khoom noj khoom haus zoo tib yam nkaus xwb. Microbiology yog ib txoj kev tshawb fawb loj heev, thiab yog li ntawd, thaum ua kev tshawb fawb, qee zaum yuav tsum tau xaiv cov kab mob me me rau qee qhov laj thawj. Kev siv cov xov xwm kev kuaj mob sib txawv hauv chav kuaj ua rau nws muaj peev xwm xaiv cov kab mob uas xav tau rau ntawm Petri tais raws li cov cim biochemical ntawm lawv cov haujlwm tseem ceeb.
Cov kev sib xyaw ntawm cov chaw ib puag ncig ib txwm suav nrog cov hauv qab no:
1. Cov zaub mov rau kev loj hlob ntawm tes.
2. Kev tshuaj xyuas substrate (substance).
3. Qhov taw qhia uas yuav muab cov xim yam ntxwv thaum muaj qee yam tshwm sim.
Ib qho piv txwv yog qhov sib txawv ntawm qhov ntsuas qhov nruab nrab "Endo". Nws yog siv los xaiv cov kab mob ntawm cov kab mob uas tuaj yeem rhuav tshem lactose. Thaum pib, qhov nruab nrab no muaj xim pinkish. Yog hais tias ib pawg ntawm cov kab mob tsis tuaj yeem rhuav tshem lactose, nws yuav siv cov xim dawb li niaj zaus. Yog tias cov kab mob tuaj yeem ua rau cov substrate no tawg tau, lawv hloov mus ua cov xim liab liab.
Hnub Wednesdays
Cov chaw kuaj mob feem ntau ua haujlwm nrog cov swabs uas muaj ntau hom kab mob sib txawv. Pom tseeb, rau kev ua haujlwm zoo, nws yog ib qho tsim nyog yuav tau xaiv cov cheeb tsam uas peb xav tau los ntawm ntau tus neeg sab nrauv. Qhov no yog qhov nruab nrab ntawm cov kab mob loj hlob tuaj yeem pab tau, uas yog tsim los pab txhawb ib hom kab mob xwb.
Piv txwv li,Xws li ib qho chaw xaiv tsuas yog tsim rau kev yug me nyuam ntawm Escherichia coli. Tom qab ntawd, los ntawm kev tseb ntau cov kab mob rau ntawm Petri tais, peb yuav pom tsuas yog cov cheeb tsam ntawm tib Escherichia coli thiab tsis muaj ntxiv lawm. Ua ntej pib ua haujlwm, nws yog ib qho tsim nyog yuav tsum paub txog cov metabolism ntawm cov kab mob uas tau kawm zoo txhawm rau txhawm rau xaiv nws los ntawm kev sib xyaw ntawm lwm hom.
Khoom, semi-kua thiab kua kab lis kev cai media
Cov kab mob tuaj yeem loj hlob tsis yog ntawm cov khoom substrates. Cov khoom noj khoom haus sib txawv ntawm ib leeg hauv lawv lub xeev ntawm kev sib sau ua ke, uas nyob ntawm qhov muaj pes tsawg leeg thaum tsim khoom. Thaum pib, lawv txhua tus muaj cov kua dej sib xws, thiab thaum gelatin lossis agar ntxiv rau hauv ib feem pua, qhov sib xyaw ua ke.
Cov kab lis kev cai ua kua dej feem ntau pom muaj nyob hauv cov hlab ntsha. Yog tias tsim nyog kom loj hlob cov kab mob nyob rau hauv cov xwm txheej zoo li no, ntxiv cov tshuaj nrog cov kab lis kev cai thiab tos 2-3 hnub. Qhov tshwm sim tuaj yeem sib txawv: cov ntaub ntawv nag lossis daus, ib zaj duab xis tshwm, me me flakes ntab, lossis cov ntaub ntawv pos huab.
Dense kab lis kev cai nruab nrab feem ntau yog siv hauv kev tshawb fawb microbiological los kawm txog cov khoom ntawm cov kab mob kab mob. Cov xov xwm zoo li no yeej ib txwm pob tshab los yog translucent kom nws muaj peev xwm txiav txim siab cov xim thiab cov duab ntawm kab lis kev cai ntawm cov kab mob.
Media npaj
Substrates xws li nqaij-peptone sib tov raws cov kua zaub, gelatin lossis agar yog yooj yim heev los npaj. Yog tias koj xav tau ua ib qho khoom lossis semi-kua substrate, ua kuaNtxiv 2-3% los yog 0, 2-0, 3% gelatin los yog agar, feem. Lawv ua lub luag haujlwm tseem ceeb hauv kev sib xyaw kom tawv nqaij, tab sis lawv tsis yog qhov chaw ntawm cov as-ham. Yog li, cov khoom noj khoom haus tau txais uas tsim nyog rau kev loj hlob ntawm kab mob kab mob.
