Selenite broth yog ib qho kev txhawb nqa nruab nrab nrog cov khoom xaiv, tsim los cais cov kab mob pathogenic ntawm genus Salmonella (lat. Salmonella). Nws yog qhov zoo heev yog tias nws yog qhov tsim nyog los kuaj xyuas cov kab mob tshwj xeeb ntawm lwm cov neeg sawv cev ntawm txoj hnyuv microflora. Qhov nruab nrab yog siv rau ob qho tib si kev kuaj mob thiab kev huv huv hauv kev sim zaub mov.
yam ntxwv dav dav
Selenite broth yog npaj los ntawm cov hmoov, uas yog homogenized sib tov ntawm ntau yam ntawm cov muaj pes tsawg leeg. Cov xim ntawm cov khoom qhuav tuaj yeem yog lub teeb daj lossis qab zib, nyob ntawm seb hom nruab nrab. Cov hmoov muaj cov khoom ntws dawb.
Npaj selenite kua zaub yog kua pob tshab nruab nrab ntawm lub teeb daj xim. Qhov loj no yog nchuav rau hauv ntim qhov chaw sowing yog nqa tawm rau incubation ntxiv.
Muaj peb yam tseem ceeb ntawm selenite broth:
- ntshiab nruab nrab - haum rau kev sib cais ntawm Salmonella los ntawm cov chaw kho mob thiab cov khoom siv huv huv;
- nrog qhov sib ntxiv ntawm mannitol (ob lub ntsiab lus broth) - tsim los ua haujlwm nrog cov khoom siv kho mob nkaus xwb;
- cysteine-selenite nruab nrab - siv tau los cais Salmonella los ntawm cov neeg mob pathological biomaterial (feces, zis, thiab lwm yam).
Cov hom no txawv me ntsis hauv kev sib xyaw thiab kev ua haujlwm.
Lub luag haujlwm tseem ceeb ntawm selenite broth yog los txhawb kev sib sau ntawm salmonella, inhibiting kev loj hlob ntawm microflora nrog. Qhov no ua rau nws muaj peev xwm tsis tsuas yog kuaj pom cov kab mob hauv cov khoom, tab sis kuj tom qab hloov mus rau agar. Qhov nruab nrab yog tsim los ntawm Leifler, uas thawj zaug nrhiav pom cov nyhuv ntawm selenite tiv thaiv Salmonella.
Composition
Selenite broth muaj:
- casein hydrolyzate;
- lactose;
- Ysodium phosphate;
- sodium hydroselenite.
Selenite-cysteine nruab nrab, ntxiv rau cov khoom no, muaj L-cysteine thiab sodium hydrogen phosphate. Cov muaj pes tsawg leeg ntawm mannitol broth txawv nyob rau hauv uas es tsis txhob ntawm casein hydrolyzate, nws muaj xws li peptic zom ntawm cov ntaub so ntswg tsiaj. Tsis muaj lactose nyob rau hauv ib puag ncig zoo li no, tab sis muaj mannitol. Lub tom kawg ua raws li fermentable substrate thiab muab lub buffering zog ntawm lub broth.
Txhua yam khoom ntawm cov hmoov, tshwj tsis yog sodium hydroselenite, raug hu ua ntu A, thiab cov khoom xaiv hu ua ntu B.
Lub hauv paus ntsiab lus ntawm kev ua haujlwm thiab cov khoom ntawm ib puag ncig
Selenite kua zaub ua haujlwm ua qhov nruab nrab cianrog rau kev xaiv. Cov nyhuv xaiv yog muab los ntawm toxicity ntawm selenite, uas inhibits kev loj hlob ntawm feem ntau cov kab mob. Nyob rau tib lub sijhawm, cov kab mob ntawm cov genus Salmonella muaj peev xwm rov qab tau cov tshuaj no, yog li tshem tawm cov tshuaj lom ntawm lawv lub hlwb. Txawm li cas los xij, raws li qhov tshwm sim ntawm cov tshuaj tiv thaiv, ib qho alkali yog tsim, uas txo cov nyhuv detrimental ntawm selenite ntawm kev loj hlob ntawm cov microflora cuam tshuam, thiab yog li nws yuav tsum ua kom ruaj khov pH. Qhov kev ua haujlwm no yog ua los ntawm cov kab mob uas ferment lactose nrog tsim cov kua qaub. Lub buffering ntawm nruab nrab yog muab los ntawm phosphate.
Nyob rau hauv ob lub ntsiab lus broth, pH yog stabilized los ntawm mannitol. Qhov nruab nrab cysteine txhim kho cov kev xaiv ntawm Salmonella. Xws li cov kua txiv hmab txiv ntoo muaj txiaj ntsig zoo rau kev tswj hwm tus kab mob keeb kwm yav dhau los ntawm cov neeg mob uas tsis muaj tus kab mob los yog tus neeg mob convalescents.
Qhov tshwm sim ntawm cov yam ntxwv ntawm kev loj hlob ntawm kev siv hom kab mob hauv nruab nrab tuaj yeem pom tom qab 12-24 teev txij li pib incubation. Hauv qhov no, Salmonella tsim cov xim tsis muaj kob.
ua noj ua haus
Kev npaj nruab nrab yog ua tiav hauv ob theem. Ua ntej, ib qho kev daws teeb meem yog ua los ntawm kev xaiv cov khoom ntawm tus nqi ntawm 4 grams ntawm sodium selenite ib liter ntawm distilled dej. Tom qab ntawd ntxiv 19 grams ntawm cov hmoov tseem ceeb (ib feem A). Cov tshuaj yog sib xyaw kom huv si thiab ua kom sov kom txog thaum cov khoom tawg tag, tom qab ntawd nws tau nchuav rau hauv cov raj uas tsis muaj menyuam.
Thaum ua noj, nws tseem ceeb heev uas yuav tsum ua rawskub tswj, txij li qhov nruab nrab tsis ruaj khov rau overheating. Sterilization ntawm broth tuaj yeem nqa tawm tsuas yog nyob rau hauv da dej los yog siv lub dav hlau ntawm chav. Autoclaving ntawm nruab nrab yog nruj me ntsis txwv.
