Hauv kev txiav txim siab sib txawv ntawm enterobacteria thiab qee qhov vibrios, Voges-Proskauer cov tshuaj tiv thaiv nyob hauv qhov chaw tshwj xeeb. Qhov kev sim no yog ua raws li qhov muaj peev xwm ntawm cov kab mob ferment qabzib los ua acetoin.
Lub ntsiab ntawm cov txheej txheem tshawb fawb
Hauv microbiology, Voges-Proskauer cov tshuaj tiv thaiv feem ntau siv los sib txawv hauv Yersinia tsev neeg ntawm enterobacteria (xws li cov kab mob ntawm pseudotuberculosis thiab enterocolitis), Escherichia coli, thiab spore-forming aerobes. Cov txiaj ntsig tau pom los ntawm kev pleev xim nruab nrab thaum qee cov tshuaj ntxiv ntxiv.
Qhov kev xeem no yog nyob rau hauv IMViC series (cov ntawv luv ntawm Indol, Methyl Red, Voges-Proskauer i Citrate) - ib pab pawg ntawm cov kev xeem txheeb xyuas suav nrog cov ntsiab lus sib txawv:
- nrog indole, raws li kev tawg ntawm tryptophan rau hauv indole, siv nyob rau hauv lub xub ntiag ntawm Kovacs los yog Ehrlich reagent;
- sivmethylroth, los yog methyl liab, uas kuaj pom pH muab los ntawm cov piam thaj metabolization;
- Voges-Proskauer cov tshuaj tiv thaiv rau kev kuaj pom ntawm acetyl-methylcarbinol;
- siv citrate nrog cov xim hloov raws li qhov tshwm sim ntawm alkalization ntawm nruab nrab.
Lub ntsiab lus ntawm cov txheej txheem yog qhov pom ntawm qhov muaj cov kab mob kuaj pom vim muaj kev cuam tshuam ntawm acetoin tsim los ntawm lawv nrog caustic potash nyob rau hauv muaj oxygen. Acetyl-methylcarbinol yog oxidized rau diacetyl, uas tsim cov xim liab los yog liab dawb. Ua kom qhov rhiab heev ntawm qhov kev sim los ntawm kev qhia alpha-naphthol ua ntej ntxiv caustic potash.
Test setting
Cov qauv ntawm Voges-Proskauer cov tshuaj tiv thaiv cuam tshuam nrog kev cog qoob loo ua ntej ntawm cov kab mob. Ib kab lis kev cai ntshiab yog sown rau ntawm Clark qhov sib txawv ntawm qhov nruab nrab, qhov sib txawv ntawm qhov uas yog Clark's broth (tsis muaj qhov sib ntxiv ntawm agar-agar). Ob qho nruab nrab npaj tau siv, lossis npaj nws tus kheej. Cov khoom xyaw muaj xws li:
- 5g peptone;
- 5g qabzib;
- 5g dibasic potassium phosphate;
- 1L distillate.
Thaum kawm, kab lis kev cai yog inoculated nrog lub voj sterile bacteriological mus rau hauv cov kua nruab nrab. Kom muaj nuj nqis - 5 ml nyob rau hauv ib lub raj kuaj ntxiv rau kev tswj. Incubation yog nqa tawm ntawm qhov kub ntawm 35-37 degrees rau ob hnub. Tom ntej no, kev kawm txog Voges-Proskauer cov tshuaj tiv thaiv yog ua nyob rau theem:
- 2, 5 ml ntawm cov kab lis kev cai yog pauv mus rau lub raj tsis muaj menyuam.
- Ntxiv rau 6 tee ntawm alpha-naphthol (5% cawv tov).
- Ntxiv 40%ib tug daws ntawm caustic poov tshuaj nyob rau hauv ib tug npaum li ntawm 0.1 ml, los yog ob tee.
- Kev sib tw yog nqa los ntawm maj mam tuav lub raj ntsuas.
- ntsuas qhov tshwm sim tom qab 15 feeb los ntawm qhov pib ntawm qhov kev sim.
Ib txoj kev sim lwm txoj hauv kev yog kev tsim tawm hmo ntuj, lub sijhawm txo qis rau 18 teev lossis txog li ib hnub. Ntxiv nrog rau txoj kev no, ib qho kev sim qhia kuj tseem siv tau: kab lis kev cai tau qhia hauv lub voj voog rau hauv 2 ml ntawm qhov nruab nrab, incubated rau li plaub teev, tom qab ntawd cov reagents ntxiv rau hauv qhov sib npaug ntawm 2-3 tee, sib tov thiab Cov txiaj ntsig tau ntsuas tom qab kaum feeb.
Kev tswj yog ib qho ntawm cov kab mob ua rau mob ntsws Klebsiella pneumoniae - strain atcc 13883.
Kev ntsuas qhov tshwm sim
Tom qab lub sijhawm xav tau tom qab ntxiv cov tshuaj reagents (los ntawm 5 mus rau 15 feeb), cov xim cherry-liab yuav tsum tau pom nrog cov lus pom zoo, liab thiab liab - nrog cov tshuaj tiv thaiv tsis muaj zog. Tsis muaj kev hloov pauv tau raug kaw vim qhov tshwm sim tsis zoo.
Ntau dhau ntawm caustic potash hauv kev daws tuaj yeem ua rau muaj kev cuam tshuam zoo, ua rau xim tooj liab. Nyob rau hauv cov ntaub ntawv no, cov lus teb tsis zoo ntawm qhov kev sim colony yuav tsum tau sau tseg. Tsis tas li ntawd, tooj liab staining tshwm nyob rau hauv rooj plaub uas qhov kev ntsuam xyuas tau muab ib teev tom qab kev qhia txog cov reagents.
Thaum ntsuas qhov tshwm sim, nws yuav tsum tau coj mus rau hauv tus account tias kev cog qoob loo ntev ntawm cov kab mob uas tau kawm (ntau tshaj peb hnub) ua rau acidification ntawm qhov nruab nrab, uas tuaj yeem ua rau qhov tsis raug ntawm txoj kev tshawb no. Cov tshuaj tiv thaiv tej zaum yuav zoo me ntsis los yogcuav negative.
Yuav tsum tau kuaj cov tshuaj tiv thaiv
Voges-Proskauer reagents yuav tsum ua kom tau raws li qhov yuav tsum tau muaj xws li qhov tseeb concentration, siab purity thiab ruaj khov, uas ua tiav los ntawm kev cia kom zoo.
Cov khoom siv npaj txhij-rau-siv feem ntau muaj cov tshuaj reagents rau 100 lossis ntau dua siv thiab tuaj rau hauv cov yas los yog iav vials. Kev ua tau zoo yog tswj hwm los ntawm cov ntaub ntawv cuam tshuam.
